Scientist, In Vitro Models

We’re seeking a scientist to design, build, and validate our “human in a dish” platform that ensures translational relevance of our preclinical development pipeline. This includes establishing and optimizing in vitro human models – such as patient-derived samples, tumor organoids, 3D cell models, organs-on-chip – to “debug” and refine our molecular programs in physiologically relevant contexts. You’ll develop immune-competent model systems to mimic the tumor microenvironment and immune interactions within healthy tissues. To support this work, you’ll build functional multi-omics workflows to profile these disease models using bulk and single-cell genomics technologies. You’ll characterize synthetic genetic components and their combinations in the context of these models to anticipate their function in patients. This role requires long-term, strategic thinking to optimize the scale-up of cell culture operations with the goal of creating a pipeline of well-validated, characterized, and qualified human cell models for high-throughput functional genomics and drug discovery studies.

Primary Responsibilities:
• Develop advanced in vitro human models of healthy and diseased primary cells, tissues, and organs to serve as a translationally relevant testing and validation platform
• Scale up culture of in vitro models to support high-throughput testing workflows
• Design assays for model characterization and to establish translational relevance
• Collaborate with our Synthetic Biology and Computational Biology teams to develop workflows for comprehensive high-throughput testing of multiple synthetic genetic components, their combinations, and therapeutic candidates
• Implement multi-modal bulk and single-cell assays as an integral part of testing workflows.
• Optimize and establish standard operating procedures for patient-derived tissue processing and dissociation
• Plan and manage complex projects including interfacing with clinical partners for sample acquisition and sequencing service providers
• Liaise with CROs, plan and oversee technology transfer and study execution at contract organizations, as needed
• Maintain detailed, well-organized documentation of experiments and data

Required Skills & Qualifications :
• PhD in molecular biology, genomics, bioengineering, immunology or a related field
• Excellent knowledge of ex vivo/in vitro models, ideally in oncology (tumor organoids, tumor explants), as well as healthy/primary human and animal cells (primary cells, stem cell models, spheroids, organoids, organotypic culture, organs-on-chip)
• Proven track record of building complex model systems including co-culture systems, 3D cellular models, or organoids
• Experience with primary tissue handling and processing
• High motivation with a strong work ethic and dedication to generating impact
• Ability to go from idea to data to conclusions in an independent manner
• Hands-on experience with tissue engineering, biomaterials, 3D bioprinting, bioreactors, or microfluidics
• Hands-on experience performing single cell and bulk genomics experiments including sample preparation, library preparation, and sequencing

At Pattern Bio, we design biological circuits at the intersection of synthetic biology and AI for cell-specific targeting of cancer tissue. By integrating data-driven design into every step—from DNA constructs to AAV-based delivery systems—we are building precise, rationally engineered, curative therapies. Our cohesive and driven team in South San Francisco is dedicated to curing cancer and saving lives.